BENZAMIDINE HYDROCHLORIDE HYDRATE

Description_x000D_ Application_x000D_ Benzamidine hydrochloride hydrate has been used:_x000D_ • in western blot analysis_x000D_ • in immunoprecipitation_x000D_ • in the purification of yeast dynein_x000D_ • as a protease inhibitor in the preparation of brush-border membrane vesicles_x000D_ • for the processing of human synovial fluid, blood and eye sleep_x000D_ • to prevent non-specific degradation of cartilages_x000D_ Packaging_x000D_ 5, 25, 100 g in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A._x000D_ In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma._x000D_ Peptidase inhibitor._x000D_ Preparation Note_x000D_ Benzamidine HCl is soluble in water and alcohol. Solubility testing in water at a concentration of 50 mg/ml yields a clear solution with heating. Benzamidine HCl is sensitive to oxidation. It is recommended to prepare solutions fresh each time in degassed water prior to use. However, frozen aliquots stored under inert gas, to exclude air, may be stable for a short time. Insufficient information is available to assess the shelf-life of a frozen solution.